ABSTRACT
O objetivo deste estudo foi avaliar o efeito da ω-conotoxina MVIIC e das células-tronco mesenquimais (CTM) de forma isolada e sua associação nos ratos submetidos ao trauma medular agudo (TMA). Trinta Rattus novergicus, linhagem Wistar, três meses de idade, foram distribuídos igualmente em cinco grupos experimentais: controle negativo (CN), controle positivo (CP), ω-conotoxina MVIIC (MVIIC), células-tronco mesenquimais da medula óssea (CTM-MO) e associação (MVIIC + CTM-MO). O grupo CN foi submetido à laminectomia sem trauma medular, e os grupos CP, MVIIC, CTM-MO e MVIIC + CTM-MO foram submetidos ao trauma medular contusivo. O grupo CP recebeu, uma hora após o TMA, 10µL de PBS estéril, e os grupos MVIIC e MVIIC + CTM-MO receberam 10µL de PBS contendo 20pmol da ω-conotoxina MVIIC, todos por via intratecal. Os grupos CTM-MO e MVIIC + CTM-MO receberam, 24 horas após, 1x106 de CTM via intravenosa. Avaliou-se a recuperação da função locomotora até o sétimo dia pós-trauma. Os animais tratados com MVIIC + CTM-MO obtiveram recuperação motora após o trauma medular agudo (P<0,05). Conclui-se que essa associação apresentou efeito neuroprotetor com melhora na função locomotora em ratos Wistar.(AU)
The objective of this study was to evaluate the effect of isolated ω-conotoxin MVIIC and mesenchymal stem cells (MSCs) and its association in rats submitted to acute spinal cord injury (SCI). Thirty Rattus norvegicus, Wistar strain, three-month-old rats were randomly distributed in five experimental groups with six animals: negative control (CN), positive control (CP), ω-conotoxin MVIIC (MVIIC), bone marrow mesenchymal stem cells (CTM-MO) and the association (MVIIC + CTM-MO). The CN group underwent laminectomy without spinal cord trauma, and groups CP, MVIIC, CTM-MO and MVIIC + CTM-MO were submitted to contusive spinal cord trauma. The CP group received 10µl of PBS one hour after SCI, and groups MVIIC and MVIIC + CTM-MO received 10µl of PBS containing 20pmol of ω-conotoxin MVIIC, both intrathecally. Groups CTM-MO and MVIIC + CTM-MO received 1x106 of MSCs intravenously 24 hours later. The recovery of locomotor function was evaluated up to seven days post-injury. The animals treated with MVIIC + CTM-MO obtained motor recovery after SCI (P<0.05). It is concluded that this association showed neuroprotective effect with improvements in locomotor function in Wistar rats.(AU)
Subject(s)
Animals , Rats , Spinal Cord Injuries/rehabilitation , Calcium Channel Blockers , omega-Conotoxins/therapeutic use , Mesenchymal Stem Cells , Cell- and Tissue-Based Therapy/veterinary , Neuroprotection , Rats, WistarABSTRACT
A redução da produção de leite em bovinos é um dos aspectos majoritários na composição total do valor de impacto econômico provocado por R. (B.) microplus. Portanto, o objetivo deste estudo foi estimar a perda decorrente da carga parasitária, observada em uma propriedade produtora de leite na região central de Minas Gerais. Para tal, foram consideradas 30 contagens de carrapatos, distribuídas de maneira uniforme durante o período de um ano, em um rebanho bovino com 74 vacas de composição racial 3/4 Holandês x Zebu, mantidas sob sistema de produção intensivo em pastoreio. A partir dos dados observados, foi possível estimar que 937.968 teleóginas completaram o repasto sanguíneo e foram responsáveis pela redução de 6.678 litros de leite durante o período avaliado. Esse valor corresponde a uma redução de 2,7% da produção, média de 90,24 litros por vaca em lactação.
Subject(s)
Animals , Female , Cattle , Damage Assessment in the Economic Sector , Economic Development , Rhipicephalus/growth & development , MilkABSTRACT
The cultivation of pejibaye (Bactris gasipaes Kunth) has been expanding in Brazil, especially in the Vale do Ribeira, SP, where the edaphoclimatic condition is compatible with its production. With the purpose of learning about insects that visit the inflorescence of pejibaye, a survey was conducted at Polo Regional do Vale do Ribeira, located in the municipality of Pariquera-açu, SP, Brazil, and on a private property located in the municipality of Registro, SP, areas where selected pejibaye palm trees from Yurimaguas, Peru, are grown. During the month of January of 2006 and 2007, yellow sticky insect traps were placed at the inflorescences of different pejibaye matrices, soon after the bracts opened. The traps were kept throughout female and male anthesis, and removed at the end of the cycle, which lasted for about 72 hours. The 9,743 insects collected were then separated, counted, and identified according to their orders. It was observed that the most frequent insects on the inflorescence of pejibaye palms in the Vale do Ribeira, SP are Diptera, Coleoptera, and Hymenoptera.
Levantamento da entomofauna associada às inflorescências de pupenheira (Arecaceae: bactris gasipaes kunth) no vale do Ribeira, SP. A cultura da pupunheira (Bactris gasipaes Kunth) vem se expandindo no Brasil, especialmente no Vale do Ribeira, SP, onde encontra condição edafoclimática compatível à sua produção. Com o objetivo de conhecer os insetos visitantes da inflorescência da pupunheira, foi realizado levantamento em duas áreas de coleção de pupunheiras selecionadas originárias de Yurimaguas, Peru, no - Polo Regional do Vale do Ribeira APTA/SAA-SP, localizado no Município de Pariquera-açu, SP, e em uma propriedade particular no Município de Registro, SP. Durante o mês de janeiro de 2006 e 2007, foram instaladas armadilhas adesivas entomológicas amarelas em inflorescências de diferentes matrizes de pupunheira logo após a abertura de suas brácteas, as quais foram mantidas durante a antese feminina e masculina e retiradas no término do ciclo, cerca de 72 horas. Efetuou-se a separação, contagem e identificação ao nível de ordem dos 9.743 insetos totais coletados. Verificou-se que os insetos mais frequentes na inflorescência da pupunheira no Vale do Ribeira, SP, pertencem às ordens Diptera, Coleoptera e Hymenoptera.
Subject(s)
Animals , Coleoptera/classification , Diptera/classification , Hymenoptera/classification , Insecta/classification , Arecaceae , Canned Heart PalmsABSTRACT
Evaluation of HIV-induced IL-2 production by peripheral blood mononuclear cells (PBMC) and HIV-specific T helper and cytotoxic T lymphocyte (CTL) responses in health care workers (HCW) occupationally exposed to HIV reveals a high rate of response to HIV among non-seroconverters. IL-10 is also known to interfere with HIV infection in vitro. To evaluate the induction of IL-10 by HIV antigens in HCW occupationally exposed to HIV, 18 HCW with percutaneous injury were enrolled in this study, 9 of them exposed to HIV-contaminated blood, and 9 exposed to HIV-negative blood. PBMC were incubated on plates coated with HIV-1 antigens, and IL-10 was measured in supernatants by ELISA. Five of nine HCW exposed to HIV-contaminated blood presented HIV-induced IL-10. Two of nine HCW exposed to HIV-negative source patients also had detectable levels of HIV-induced IL-10, one of them in the sample obtained on the day of accidental exposure. There was a relationship between the type of device involved in injury and IL-10 production. Individuals exposed to hollow needles or scalpels presented HIV-induced IL-10, whereas those exposed to solid needles and to digital puncture did not, suggesting a relationship between infectious load and IL-10. Although occupational exposure to HIV leads to a low rate of seroconversion, these individuals can develop an antigen-specific immune response characterized in our study by induction of IL-10 in PBMC in vitro
Subject(s)
Humans , Accidents, Occupational , Health Personnel , HIV-1 , Interleukin-10 , Leukocytes, Mononuclear , Occupational Exposure , HIV Infections , HIV Seronegativity , HIV Seropositivity , Infectious Disease Transmission, Patient-to-Professional , Interleukin-10 , Leukocytes, Mononuclear , Needlestick InjuriesABSTRACT
Sixteen patients acutely poisoned with aldrin were examined to evaluate a possible correlation between serum aldrin and diedrin levels and clinical complaints. The patients were classified as having mild (N = 8), moderate (N = 5) or severe (N = 3) poisoning according to clinical symptoms. Concentrations of less than 20 µg/l were usually associated with mild poisoning, which involved complaints such as nausea, vomiting and epigastric pain, whereas concentrations of 100 to 200 µg/l were considered to represent moderate intoxication and were associated with nausea, vomiting, epigastric pain, headache, dizziness, and convulsions. Sever or fatal cases were associated with levels above 700 µg/l. Taken together,these results suggest that serum aldrin diedrin levels can be used as indicators of clinical prognosis after acute poisoning with these insecticides and that convulsions could suddenly occur even in the absence of prodronal signs or symptoms